Journal: Nature Communications

Article Title: Double-strand breaks in facultative heterochromatin require specific movements and chromatin changes for efficient repair

doi: 10.1038/s41467-024-53313-2

Figure Lengend Snippet: A Cell culture set up to analyze the dynamics of ATRIP localization upon DSB induction using 5Gy gamma-irradiation. Figure partly created in BioRender. Janssen, A. (2023) BioRender.com/o68s986 and Janssen, A. (2023) BioRender.com/v72e853. B Representative images of ATRIP-eYFP focus (green) dynamics within the ph-p-mCherry domain (magenta). Arrow indicates ATRIP-eYFP focus arising within ph-p-mCherry domain (0 min) and moving outside this domain (10 min). Scale bar = 2 μm. Dotted lines outline nuclei. C Quantification of ATRIP-eYFP (DSB) localization 10 min after ATRIP-eYFP appearance, 3 biological replicates per condition (yellow control dsRNA or dUtx dsRNA). Colors indicate localization of the ATRIP-eYFP focus (inside polycomb body [purple], outside [green], not detectable or resolved [gray]). p-value: 0.0490. D Relative dUtx expression level in Drosophila Kc cells normalized to an internal control gene (tubulin), determined using RT-qPCR. Bars indicate averages +SD of 3 biological replicates per condition. p-value: 0.0053. E Quantification of the number of cells with an ATRIP-eYFP focus inside ph-p-mCherry domain divided by the total number of cells expressing both ATRIP-eYFP and ph-p-mCherry. Each dot indicates the percentage of cells with ATRIP foci overlapping with a ph-p domain per acquired image (total of 20 images (control) and 18 images (dUtx dsRNA) were aquired during 3 biological replicates). Averages +SEM are shown. p-value: 0.0289. (ns) not significant, (*) p-value ≤ 0.05, (**) p-value ≤ 0.01, two-sided Chi-square test ( C ), two-sided paired t-test ( D ) and two-sided unpaired t-test ( E ). Source data are provided as Source Data file.

Article Snippet: To prepare trimethoprim containing food, 1.67 g instant Drosophila medium (Formula 4–24, Carolina Biological Supply) was mixed with 5 mL non-distilled water containing 5.3 μL of 100 mM trimethoprim while vortexing.

Techniques: Cell Culture, Irradiation, Control, Expressing, Quantitative RT-PCR

Journal: Cell reports

Article Title: Wnd/DLK Is a Critical Target of FMRP Responsible for Neurodevelopmental and Behavior Defects in the Drosophila Model of Fragile X Syndrome

doi: 10.1016/j.celrep.2019.08.001

Figure Lengend Snippet:

Article Snippet: Formula 4-24 Instant Drosophila Medium Blue Food , Carolina Biological Supply , Cat# 173210.

Techniques: Recombinant, cDNA Synthesis, SYBR Green Assay, Protease Inhibitor, BIA-KA, Software